Detail Hepatic Oval Cells Have the Side Population Phenotype Defined by Expression of ATP-Binding Cassette Transporter ABCG2/BCRP1 (Short Communication in American Journal of Pathology, Vol. 163, No. 1) Bibliografi Author: Shimano, Koichi ; Satake, Makoto ; Okaya, Atsuhito ; Kitanaka, Junichi ; Kitanaka, Nobue ; Takemura, Motohiko ; Sakagami, Masafumi ; Terada, Nobuyuki ; Tsujimura, Tohru Topik: Short Communication; Side Population (SP) Phenotype; Hoechst 33342 Exclusion Assay; Rat ABCG2/BCRP1; Parenchymal and Nonparenchymal Cells; Northern Blot Analysis; In Situ Hybridization Bahasa: (EN )    Edisi: Jul 2003     Penerbit: American Society for Investigative Pathology     Tempat Terbit: Bethesda    Tahun Terbit: 2003     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 3.pdf (421.84KB; 0 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Organ-specific stem cells can be identified by the side population (SP) phenotype, which is defined by the property to effectively exclude the Hoechst 33342 dye. The ATP-binding cassette transporter ABCG2/ BCRP1 mediates the SP phenotype. Because hepatic oval cells possess several characteristics of stem cells, we examined whether they have the SP phenotype using the 2-acetylaminofluorene/partial hepatectomy (PH) model. Fluorescence-activated cell sorting analysis showed that a population of non-parenchymal cells containing oval cells, prepared on day 7 after PH, carried a significant number of SP cells, whereas that of non-parenchymal cells without oval cells, prepared on day 0 after PH, did not. Northern blot analysis using total liver RNA obtained on various days after PH showed that the expression of ABCG2/BCRP1 mRNA increased after PH, reaching the highest level on day 7, and then gradually decreased. This pattern of changes in the ABCG2/BCRP1 mRNA level was well correlated to that in the number of oval cells. Furthermore, in situ hybridization revealed that oval cells were the sites of expression of ABCG2/BCRP1 mRNA. These results indicate that oval cells have the SP phenotype defined by expression of ABCG2/BCRP1, suggesting that oval cells may represent stem cells in the liver. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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