Detail The Development of Multiplex Flow Cytometry Assay to Detect Dengue Virus Bibliografi Author: Sugiharto, Victor Arief ; Lay, Bibiana Widiyati (Advisor); Burgess, Timothy H. (Advisor); WIDJAJA, SUSANA (Advisor) Topik: Multiplex Assay; Antigen Detection; Dengue Bahasa: (EN )     Penerbit: Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)     Tempat Terbit: Jakarta    Tahun Terbit: 2008     Jenis: Theses - Undergraduate Thesis Fulltext: Victor Arief Sugiharto's Undergraduate Theses.pdf (514.83KB; 3 download) Ketersediaan Perpustakaan Pusat (Semanggi) Nomor Panggil: FTB-099 Non-tandon: tidak ada Tandon: 1  Lihat Detail Induk Abstract Dengue infection is highly endemic in Indonesia with increasing incidence rate every year. Dengue treatments are usually given symptomatically and essential to increase the patient’s survival rate if given in early phase of infection. Accurate and rapid dengue virus detection is important to diagnose and treat dengue patient. A multiplex assay (Luminex technology) is developed to detect dengue viruses. Monoclonal antibody from mouse ascitic fluid and hybridoma supernatant were produced and used in this study. It was shown that incomplete Freund’s adjuvant (IFA) gave better ascites production compared to pristane. The usage of higher hybridoma cells injected also gave higher volume of ascites. However, an optimum injection condition has to be generalized for each hybridoma. Luminex assay using 2.5 µg of 3H5 monoclonal antibody could detect 5x103 PFU dengue-2 (DEN-2) virus stock while 2.5 µg of 1H10 monoclonal antibody could detect 75 PFU dengue-4 (DEN-4) virus stock. Further coupled antibody and reporter optimization needs to be done to increase the sensitivity of DEN-2 and DEN-4 detection. The usage and optimization of anti-DEN-1 and anti-DEN-3 monoclonal antibody needs to be done in order to develop a multiplex assay for dengue diagnosis. Luminex assay is an alternative laboratory technique to diagnose dengue infection rapidly and cost-effectively. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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