A novel binding site of a mutant (hLTB/S4N) of E.coli heat-labile enterotoxin (LT) B subunit from human isolates, was observed in previous research (Holmner et al., 2004, Structure, 12: 1655-67). The site was speculated to come from an ancestor of hLTB. In order to reveal the evolutionary history of the novel binding site of the protein (hLTB/S4N), evolutionary trace method was applied. The results and limitations of this study are described. A subsequent analysis of the sites around fucose molecules in 109 structures from the Protein Data Bank is presented. Some structural
characteristics of the sites were observed. |