This thesis deals with a computational comparison of two experimental methods used to
perform large-scale analysis of quantitative phenotypes in deletion mutants of Saccharomyces
cerevisiae. The two methods are the ‘Barcode’ method developed by the Ron Davis Group
(at Stanford Univ., USA), and the ‘Microcultivation’ method used by the Anders Blomberg
group (at Göteborg Univ., Sweden). In both cases, large-scale, gene-deletion mutants of
S.cerevisiae are grown and their ability to survive and grow in different environmental
conditions is studied.
This thesis compares the two methods by analyzing whether the growth phenotypes obtained
in the Microcultivation method can be determined using the Barcode method, and to what
degree of accuracy. The growth phenotypes analyzed here were the length of the adaptation
time (length of the Lag phase) and the rate of Growth (slope of the Exponential phase).
An analysis is also done on the effect of the technical error on these growth variables. |