Detail Quantitative and Cost Comparison of Ultrasensitive Human Immunodeficiency Virus Type 1 RNA Viral Load Assays:Bayer bDNA Quantiplex Versions 3.0 and 2.0 and Roche PCR Amplicor Monitor Version 1.5 (in Journal of Clinical Microbiology Vol. 38, No. 3) Bibliografi Author: Elbeik, Tarek ; Ng, Valerie ; Charlebois, Edwin ; Nassos, Patricia ; Kahn, James ; Hecht, Frederick M. ; Yajko, David ; Hadley, Keith Topik: Human Immunodeficiency Virus type 1 (HIV-1) RNA; Highly Active Anti-Retroviral Therapy (HAART); ultrasensitive assays; the Bayer Quantiplex HIV-1 bDNA version 3.0 (bDNA 3.0) assay; the Roche Amplicor HIV-1 Monitor Ultrasensitive version 1.5 (Amplicor 1.5) assay Bahasa: (EN )    Edisi: Maret 2000     Penerbit: American Society for Microbiology     Tempat Terbit: Washington, DC    Tahun Terbit: 2000     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Bayer (Quantitative...pdf (115.12KB; 1 download) Abstract Quantification of human immunodeficiency virus type 1 (HIV-1) RNA as a measure of viral load has greatly improved the monitoring of therapies for infected individuals. With the significant reductions in viral load now observed in individuals treated with highly active anti-retroviral therapy (HAART), viral load assays have been adapted to achieve greater sensitivity. Two commercially available ultrasensitive assays, the Bayer Quantiplex HIV-1 bDNA version 3.0 (bDNA 3.0) assay and the Roche Amplicor HIV-1 Monitor Ultrasensitive version 1.5 (Amplicor 1.5) assay, are now being used to monitor HIV-1-infected individuals. Both of these ultrasensitive assays have a reported lower limit of 50 HIV-1 RNA copies/ml and were developed from corresponding older generation assays with lower limits of 400 to 500 copies/ml. However, the comparability of viral load data generated by these ultrasensitive assays and the relative costs of labor, disposables, and biohazardous wastes were not determined in most cases. In this study, we used matched clinical plasma samples to compare the quantification of the newer bDNA 3.0 assay with that of the older bDNA 2.0 assay and to compare the quantification and costs of the bDNA 3.0 assay and the Amplicor 1.5 assay. We found that quantification by the bDNA 3.0 assay was approximately twofold higher than that by the bDNA 2.0 assay and was highly correlated to that by the Amplicor 1.5 assay. Moreover, cost analysis based on labor, disposables, and biohazardous wastes showed significant savings with the bDNA 3.0 assay as compared to the costs of the Amplicor 1.5 assay. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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