Detail Serum proteomic analysis focused on fibrosis in patients with hepatitis C virus infection (in Journal of Translational Medicine 2007, 5:33) Bibliografi Author: Cutler, Paul ; White, Ian R. ; Patel, Keyur ; Symonds, William T. ; Dev, Anouk ; Griffin, Philip ; Tsokanas, Nikos ; Skehel, Mark ; Chiang, Liu ; Zekry, Amany ; Gattu, Mahanandeeshwar ; Rockey, Don C. ; Berrey, Michelle M. ; McHutchison, John G. (Co-Author) Topik: Serum Proteomic Analysis; Fibrosis; Hepatitis C Virus; Liver Biopsy Bahasa: (EN )    Edisi: July 2007     Penerbit: BioMed Central     Tempat Terbit: London    Tahun Terbit: 2007     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 1479-5876-5-33.pdf (437.9KB; 1 download) [Informasi yang berkaitan dengan koleksi ini di internet] Abstract Background: Despite its widespread use to assess fibrosis, liver biopsy has several important drawbacks, including that is it semi-quantitative, invasive, and limited by sampling and observer variability. Non-invasive serum biomarkers may more accurately reflect the fibrogenetic process. To identify potential biomarkers of fibrosis, we compared serum protein expression profiles in patients with chronic hepatitis C (CHC) virus infection and fibrosis. Methods: Twenty-one patients with no or mild fibrosis (METAVIR stage F0, F1) and 23 with advanced fibrosis (F3, F4) were retrospectively identified from a pedigreed database of 1600 CHC patients. All samples were carefully phenotyped and matched for age, gender, race, body mass index, genotype, duration of infection, alcohol use, and viral load. Expression profiling was performed in a blinded fashion using a 2D polyacrylamide gel electrophoresis/LC-MS/MS platform. Partial least squares discriminant analysis and likelihood ratio statistics were used to rank individual differences in protein expression between the 2 groups. Results: Seven individual protein spots were identified as either significantly increased (a2-macroglobulin, haptoglobin, albumin) or decreased (complement C-4, serum retinol binding protein, apolipoprotein A-1, and two isoforms of apolipoprotein A-IV) with advanced fibrosis. Three individual proteins, haptoglobin, apolipoprotein A-1, and a2-macroglobulin, are included in existing non-invasive serum marker panels. Conclusion: Biomarkers identified through expression profiling may facilitate the development of more accurate marker algorithms to better quantitate hepatic fibrosis and monitor disease progression. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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