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Thidiazuron influences the endogenous levels of cytokinins and IAA during the flowering of isolated shoots of Dendrobium (in Journal of Plant Physiology 163 (2006) 1126—1134)
Bibliografi
Author:
Kerbauy, Gilberto Barbante
;
Ferreira, Wagner de Melo
(Co-Author);
Kraus, Jane Elizabeth
;
Pescador, Rosete
;
Suzuki, Rogerio Mamoru
Topik:
Auxin
;
Cytokinins
;
Dendrobium
;
Flowering
;
Orchid
Bahasa:
(EN )
Tahun Terbit:
2006
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
tdz dendro.pdf
(636.86KB;
1 download
)
Abstract
This study reports the effects of thidiazuron (TDZ) on the endogenous levels of indoleacetic acid (IAA), zeatin, zeatin riboside ([9R]Z), isopentenyladenine and isopentenyladenosine ([9R]iP) as well as structural changes in the apical meristem of Dendrobium Second Love shoots during flower induction and initial development in vitro. The results revealed that the presence of 1.8 µM TDZ had a profound effect on the endogenous cytokinins (CKs) and IAA levels of the explants, when compared to those grown on a TDZ-free medium. A significant increase in CKs (especially [9R]iP and [9R]Z) and IAA in the first samples (taken at day 5) grown on TDZ-enriched medium was associated with flower induction, while a second increase in the level of these hormones after 25 d of culture was related to flower development. The histological changes detected in the shoot apical meristem of explants grown in the presence of 1.8 µM TDZ during 30 d of culture are also described. Based on these findings, it is suggested that both auxin and CKs seem to be involved with the floral transition of
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