Detail Plasmid-Dependent Attachment of Agrobacterium tumefaciens to Plant Tissue Culture Cells (in Infection and Immunity, Nov. 1978, p. 516-522) Bibliografi Author: Matthysse, Ann G. ; Wyman, Pat M. ; Holmes, Kathryn V. Topik: Ti (tumor inducing)-plasmid; Agrobacterium; avirulent strains; tobacco; carrot Bahasa: (EN )     Tahun Terbit: 1978     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: plasmid attachment.pdf (1.65MB; 0 download) Abstract Kinetic, microscopic, and biochemical studies show that virulent Ti (tumor inducing)-plasmid-containing strains of Agrobacterium attach to normal tobacco and carrot tissue culture cells. Kinetic studies showed that virulent strains of A. tumefaciens attach to the plant tissue culture cells in increasing numbers during the first 1 to 2 h of incubation of the bacteria with the plant cells. Five Ti-plasmid containing virulent Agrobacterium strains showed greater attachment to tobacco cells than did five avirulent strains. Light and scanning electron microscopic observations confirmed that virulent strains attached to the surface of carrot cells while avirulent strains showed little attachment. Bacterial attachment was blocked by prior incubation of the plant cells with lipopolysaccharide extracted from A. tumefaciens, but not from A. radiobacter, suggesting that bacterial lipopolysaccharide is one of the components involved in the attachment process. At least one other bacterial product may be required for attachment in tissue culture because the avirulent A. tumefaciens NT1, which lacks the Ti plasmid, does not itself attach to tobacco cells, but its lipopolysaccharide does inhibit the attachment of virulent strains. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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