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Integrated Bombardment and Agrobacterium Transformation System: an Alternative Method for Soybean Transformation (Protocols in Plant Molecular Biology Reporter 18: 51–59, 2000)
Bibliografi
Author:
Droste, Annette
;
Pasquali, Giancarlo
;
Bodanese-Zanettini, Maria Helena
Topik:
Agrobacterium
;
Glycine max
;
particle bombardment
;
somatic embryogenesis
;
transformation
Bahasa:
(EN )
Tahun Terbit:
2000
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
r00-010.pdf
(49.19KB;
2 download
)
Abstract
Described here is a new method for soybean transformation, based on microwounding of embryonic clumps by particle bombardment prior to inoculation with an Agrobacterium suspension. The method combines the advantages of somatic embryogenesis and gene transfer through an integrated transformation system. This is the first report of application of this technique to transformation of Glycine max.
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