Detail Induction of early flowering in Cymbidium niveo-marginatum Mak in vitro (in Plant Cell Reports (1999) 19: 1–5) Bibliografi Author: Kostenyuk, I. ; Oh, B.J. ; So, I.S. Topik: Orchid; In vitro flowering; Phytohormones; Nutrient application; Root excision Bahasa: (EN )     Tahun Terbit: 1999     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: k55f90w8yc5pv0cn[1].pdf (211.5KB; 0 download) Abstract Many orchids take several years to flower. We have been able to induce early flowering in the temperate orchid Cymbidium niveo-marginatum Mak in vitro. The combined treatment of cytokinin (6- benzylaminopurine), restricted nitrogen supply with phosphorus enrichment, and root excision (pruning) induced transition of the Cymbidium shoot from a vegetative to a reproductive stage. Nearly 100% of the plants flowered within 90 days only when the combined treatment was applied. When root excision and/or 6- benzylaminopurine were omitted from the combined treatments, flower induction was significantly reduced. The auxin transport inhibitor, 2,3,5-triiodobenzoic acid prevented flowering of Cymbidium in vitro, although auxin (a-naphthaleneacetic acid) itself did not induce flowering. Gibberellic acid markedly delayed flowering in C. niveo-marginatum even when the flowerpromoting treatment was applied. Paclobutrazol, an anti-gibberellin agent, totally blocked the inductive effects of either cytokinin or pruning. These observations suggest that concerted actions of auxin, cytokinin, and gibberellin, as well as nutrient concentration and putative promoting/suppressing agents, determine the timing of Cymbidium orchid transition from the vegetative to reproductive stage. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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