Detail In vitro propagation of Vanilla planifolia, a tropical orchid (Research Communications in Current Science (2000) Vol. 79, No. 6) Bibliografi Author: Geetha, S. ; Shetty, Sudheer A. Topik: In vitro propagation; Vanilla planifolia; shoot tip; nodal segments; micropropagation; orchid Bahasa: (EN )     Tahun Terbit: 2000     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: INVITROPROP-VANILLA.pdf (100.21KB; 2 download) Abstract A simple and efficient micropropagation protocol was developed for Vanilla planifolia using shoot tip and nodal segments as explants. The explants were cultured in MS basal medium with 1 mg/l BAP for 10 weeks and subcultured onto fresh medium every 4 weeks. The proliferating clusters were cultured in N69 basal medium with BAP (0.5 mg/l) + d-biotin (0.05 mg/l) + folic acid (0.5 mg/l) and 2% sucrose for elongation of shoots, formation of root initials and further proliferation of axillary shoots. Separation and culturing of elongated shoots in fresh medium for 2 to 3 weeks yielded 7 to 8 cm long plantlets, which were acclimatized in polytunnels. The clusters of shoots, which did not elongate, served as a continuous source of explants for multiplication. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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