Detail In vitro propagation of Cryptocoryne wendtii (in Aquatic Botany 63 (1999) 197-202) Bibliografi Author: Kane, Michael E. ; Davis, Greg L. ; McConnell, Dennis B. ; Gargiulo, Jennifer A. Topik: Araceae; Aquarium plants; Growth regulators; Micropropagation; Plant tissue culture; Shoot culture Bahasa: (EN )     Tahun Terbit: 1999     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: KaneCryptocoryne[2].pdf (222.95KB; 2 download) Abstract Procedures for in vitro establishment, axillary shoot proliferation and plantlet acclimatization of the aquatic plant, Cryptocoryne wendtii De Wit. were determined. Surface-sterilized rhizome shoot tips were established on a basal medium (BM) consisting of Murashige-Skoog mineral salts, 0.56 mM myo-inositol, 1.2 µM thiamine-HCL and 87.6 mM sucrose supplemented with 2.2 µM N6-benzyladenine (BA) and 0.57 µM indole-3-acetic acid (IAA) and solidified with 0.8% TC agar. Effects of basal medium supplementation with factorial combinations of BA (0-25 µM) and IAA (0-10 µM) on axillary shoot proliferation from single-node explants were determined after 28 days. Maximum axillary shoot proliferation (sevenfold increase) occurred on medium supplemented with 20 µM BA alone. Excellent microcutting rooting (100%) was achieved by direct sticking microcuttings in Metro Mix 500 soilless planting medium. Greenhouse acclimatization of rooted microcuttings was 100%. High-quality salable plants were produced within eight weeks posttransplant. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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