Detail Green Fluorescent Protein as an All-purpose Reporter in Petunia (in Plant Molecular Biology Reporter 18: 219–226, 2000) Bibliografi Author: Garabagi, Freydoun ; Strommer, Judith Topik: adhl; adh2; CaMV 35S promoter (35S); green fluorescent protein; Petunia hybrida; pollen; transmitting tract Bahasa: (EN )     Tahun Terbit: 2000     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: GFP-Petunia.pdf (1.01MB; 27 download) Abstract Two critical attributes of a reporter gene are ease of scoring for activity and capacity for expression in all cell types. We have examined a variant of the gene encoding green fluorescent protein, mgfp5, for its ability to meet these criteria in petunia. Under regulation of the Cauliflower Mosaic Virus (CaMV) 35S promoter, GFP was detectable in all vegetative and most floral cell types. Promoters from petunia adhl and adh2 allowed for production of GFP in those few cell types lacking GFP production from the CaMV 35S promoter, verifying its capacity for expression in all cell types. With the appropriate promoter, GFP fluorescence was thus readily detectable throughout the plant. A potential complication is the green autofluorescence exhibited by some plant tissues. This autofluorescence is for the most part distinguishable from that contributed by GFP, but underscores the need for appropriate controls in GFP-reporter-based experiments. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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