Detail Effect of Phosphate on Cell Growth and Polysaccharide Production by Suspension Cultures of Protocorm-like Bodies of Dendrobium huoshanense (in Chin J Biotech, 2006, 22(4), 613-618) Bibliografi Author: Jiang Shao-Tong ; Wei, Ming ; Luo, Jian-Ping Topik: Dendrobium huoshanense; protocorm-like bodies; suspension culture; initial phosphate concentrations; polysaccharides Bahasa: (EN )     Tahun Terbit: 2006     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: phospate-polysakarida-protokorm.pdf (260.85KB; 0 download) Abstract The effect of inorganic phosphate on the cell growth and accumulation of polysaccharides together with nutrient utilization was investigated in suspension cultures of protocorm-like bodies of Dendrobium huoshanense. Thirty-day-old cells were transferred into a liquid medium with the inoculum density of 100 g/L cells. The results indicated that the optimal concentration of phosphate in the medium for the growth of protocorm-like bodies of Dendrobium huoshanense was 2.5 mmol/L and the biomass was 496.5 g/L (fresh weight). The phosphate was a limiting factor for the cell growth and there was a relationship between the levels of intracellular phosphate and the cell growth. A total of 2.5 mmol/L of medium phosphate was beneficial to the absorption of carbon and nitrogen source. A total of 0.312 mmol/L of medium phosphate was better for the accumulation of polysaccharides and the production of polysaccharides was 2.22 g/L on day 36. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

Copyright © 2006, 2007 Unika Atma Jaya, all rights reserved