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Detail
BukuCallus induction and somatic embryogenesis of Phalaenopsis (in Plant Cell Reports (1998) 17: 446–450)
Bibliografi
Author: Ishii, Y. ; Takamura, T. ; Goi, M. ; Tanaka, M.
Topik: Phalaenopsis; Protocorm-like bodies; Callus induction; Somatic embryogenesis
Bahasa: (EN )    
Tahun Terbit: 1998    
Jenis: Article - diterbitkan di jurnal ilmiah internasional
Fulltext: Callus induction.pdf (390.16KB; 3 download)
Abstract
Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer ‘Santa Cruz’ were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g · l–1. Medium containing 200 ml · l–1 coconut water together with 40 g · l–1 sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.
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