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Shoot regeneration via direct organogenesis from in vitro derived leaves of mulberry using thidiazuron and 6-benzylaminopurine (in Scientia Horticulturae 106 (2005) 593–602)
Bibliografi
Author:
Chitra, D.S. Vijaya
;
Padmaja, G.
Topik:
Direct shoot organogenesis
;
Leaf explants
;
Morus indica L.
;
Morus alba L.
;
Thidiazuron
;
6-benzylaminopurine
Bahasa:
(EN )
Tahun Terbit:
2005
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
mulberry.pdf
(96.29KB;
2 download
)
Abstract
A brief culture of mulberry leaves for 8–10 days on MS medium with 18.17 µM TDZ followed by transfer to 8.88 µM BAP supplemented medium triggered high frequency shoot organogenesis (77.6–89.2%) and favoured shoot elongation in Morus spp. Shoot proliferation was highest in the presence of 2.22 µM BAP with induction of 9.4–10.6 shoots per culture. High frequency of root induction (76.0–86.6%)was observed on medium supplemented with 0.49 µMIBA whereas increase in the level of IBA (4.92 µM) resulted in induction of roots along with development of callus from the base of the shoots.The regeneratedplants establishedinsoil at higher frequency in rainy season compared to winter and summer.
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