Detail In Vitro Flowering and Shoot Multiplication from Nodal Explants of Ceropegia bulbosa Roxb. var. bulbosa (in Taiwania, 48(2): 106-111) Bibliografi Author: Britto, S. John ; Natarajan, E. ; Arockiasamy, D. I. Topik: Ceropegia bulbosa Roxb. var. bulbosa; Medicinal plant; Micropropagation; In vitro flowering. Bahasa: (EN )     Tahun Terbit: 2003     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: dl_id=36.pdf (75.81KB; 0 download) Abstract A rapid micropropagation system was developed for Ceropegia bulbosa Roxb. var. bulbosa. First five nodes of ~ 1.0 cm each, harvested from young healthy shoots from garden raised plants were cultured on B5 medium supplemented with different concentrations of BA and AdS each in combination with 0.05 mg/L NAA. Multiple shoot formation of upto 12 shoots was observed within one week in presence of BAP (3 mg/L) and NAA (0.05 mg/L). Shoots were multiplied by subculture on the same medium. Shoots of 3-4 cm length were rooted in medium supplemented with 2 mg/L IBA. The rooted plantlets were hardened and successfully established in pots at 70% success rate. In vitro flowering was observed at 0.5 mg/L BA+1 mg/L GA3. Shoots transferred to the medium containing kinetin (0.05 mg/L) + IBA (2 mg/L) showed microtubers in 28 days while the shoots cultured in presence of 1 mg/L GA3 +0.5 mg/L of BA showed 76% flowering leading to seed production and 65% of the seeds germinated in successive generations. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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