Detail 16S rRNA Gene Sequencing and the Routine Clinical Microbiology Laboratory: a Perfect Marriage? (Letters to The Editor in Journal of Clinical Microbiology, Sept. 2006, p. 3469–3470) Bibliografi Author: Boudewijns, Michael ; Bakkers, Judith M. ; Sturm, Patrick D.J. ; Melchers, Willem J.G. Topik: 16S rRNA Gene Sequencing; Genotypic method; Microbiological Bahasa: (EN )     Penerbit: American Society for Microbiology     Tempat Terbit: Washington, DC    Tahun Terbit: 2006     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 3469.pdf (37.09KB; 3 download) Abstract In the December 2005 issue of the Journal of Clinical Microbiology, Petti et al. reported three cases of endocarditis in which 16S rRNA gene sequencing revealed phenotypic misidentification of the bacterial isolates (10). The authors argued in favor of implementation of 16S rRNA gene sequencing in the clinical laboratory, stating that this genotypic method is more objective, accurate, and reliable than phenotypic methods. Regarding potential flaws, they mentioned only that 16S rRNA gene sequencing is not a perfect method. On the other hand, they clearly listed the many drawbacks of phenotypic methods. Although we do not argue against 16S rRNA gene sequencing, we believe that the opinion of the authors is too optimistic and does not always reflect the daily practice in a routine clinical microbiological laboratory. Drawbacks and pitfalls of 16S rRNA gene sequencing are wellknown and have been reviewed elsewhere (6, 9). However, there has been less attention to the obstacles towards 16S rRNA gene sequencing from the routine clinical microbiology laboratory’s perspective. From our daily experiences with 16S rRNA gene sequencing, we want to comment on what we believe to be the major problems regarding the implementation of 16S rRNA gene sequencing in routine practice. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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