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Effect of various polyamines on production of protocorm-like bodies in orchid—Dendrobium ‘Sonia’ (in Scientia Horticulturae 100 (2004) 161–168)
Bibliografi
Author:
Saiprasad, G.V.S.
;
Raghuveer, P.
;
Khetarpal, S.
;
Chandra, R.
Topik:
Somatic embryogenesis
;
Regeneration
;
Putrescine
;
Spermidine
;
Spermine
;
Methane
Bahasa:
(EN )
Tahun Terbit:
2004
Jenis:
Article - diterbitkan di jurnal ilmiah internasional
Fulltext:
polyamin-protocorm.pdf
(177.86KB;
0 download
)
Abstract
Polyamines, putrescine, spermidine and spermine all at three concentrations viz., 0.2, 0.4 and 1.0mM were tested, by supplementing to MS basal + 4.44µm BAP medium. The explant used was fractionated plb. Ethylene and methane was measured at 20 and 40 days after inoculation (DAI). Among various polyamines tested, maximum number of plb’s (protocorm-like bodies) were produced in putrescine 0.4mM treatment. Increase (1.0 mM) or decrease (0.2 mM) in concentration caused a decrease in the production of plb’s. All spermidine and spermine treatments resulted in the production of less number of plb’s than control. No ethylene evolution was observed in any of the polyamine treatments. However, methane evolution was observed in all the polyamine treatments. The absolute amounts, of methane evolved could not be related to the observed plb’s production response. However, when the evolution of methane was more than 1 nmol per gram FW h-1, poor plb’s production was observed.
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