Detail Cloning of a-amylase Genes from Indonesian isolates of Thermophilic Bacteria Bibliografi Author: Nuryanto, Tommy ; Suwanto, Antonius (Advisor) Topik: Cloning; a-amylase; thermophilic bacteria Bahasa: (EN )     Penerbit: Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)     Tempat Terbit: Jakarta    Tahun Terbit: 2007     Jenis: Theses - Undergraduate Thesis Fulltext: Tommy Nuryanto's Undergraduate Theses.pdf (731.04KB; 13 download) Ketersediaan Perpustakaan Pusat (Semanggi) Nomor Panggil: FTB-081 Non-tandon: tidak ada Tandon: 1  Lihat Detail Induk Abstract A 1.6-kb DNA fragment containing a-amylase gene from Bacillus SK1 and Geobacillus tearothermophilus L-07 have been amplified using a pair of new primers designed from Geobacillus stearothermophilus a-amylase coding sequences. The fragments were ligated into a cloning vector and transformed into E. coli DH5a. The Recombinant plasmids (pTN101 & pTN201) were isolated and digested with restriction endonucleases EcoRI to verify the presence of insert DNA. The digestion result showed that each recombinant plasmids carry insert DNA fragment with the correct size. The insert DNA was subsequently sequenced, and nucleotide sequence alignment for SK1 and L-07 showed 99% similarities to the known Bacillus stearothermophillus gene encoding maltohexaoseproducing a-amylase and Bacillus stearothermophilus DNA encoding a-amylase, respectively. For construction of recombinant mesophilic Bacillus carrying thermophilic a- amylase gene, pTN101 and pTN201 were digested with restriction endonucleases NdeI and BglII to isolate the insert DNA. Those insert DNA were ligated into the Escherichia/Bacillus shuttle vector and transformed into E. coli DH5a. NdeI and BamHI were used to verify the presence of insert DNA in Escherichia/Bacillus shuttle vector. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

Copyright © 2006, 2007 Unika Atma Jaya, all rights reserved