Detail Factors affecting delivery and transient expression of-glucuronidase gene in Dendrobium Sonia protocormlike-body (in African Journal of Biotechnology Vol. 5 (2), pp. 88-94, 16 January 2006) Bibliografi Author: Janna OA ; Maziah M. ; Parveez G.K.A., Ahmad ; Saleh K. Topik: biolistic transformation; orchid; Dendrobium; protocorm like body; glucuronidase assay. Bahasa: (EN )     Tahun Terbit: 2006     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: factor expression b-gal-Dendro sonia.pdf (632.08KB; 0 download) Abstract The effect of the biolistic device parameters and other factors affecting delivery and expression of uidA gene in Dendrobium Sonia was investigated. Three week old protocorm like body (PLB) were bombarded with gold microparticles coated with pAHC25 plasmid harbouring the uidA gene which encodes -glucuronidase. The factors investigated were the helium pressure, target distance, macrocarrier flight distance to stopping screen, distance from stopping screen to target tissues, vacuum pressure, gold microparticles size, spermidine and calcium on DNA precipitation, and the number of bombardments. Two days after bombardment, the PLB were subjected to histochemical GUS assay, and transient GUS activity was recorded as blue spots using a Leica stereomicroscope. All the factors tested showed significant effects (p<0.05) on the delivery of DNA and expression of the uidA gene in Dendrobium PLB except for calcium. Surviving PLBs were able to grow and regenerate normally into plantlets. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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