Detail Development of Transformation System of Rice Based on Binary Bacterial Artificial Chromosome (BIBAC) Vector (in Actu Geneticu Sinica, March 2006, 33 (3): 269-276) Bibliografi Author: He, Rui-Feng ; Wang, Yuan-Yuan ; DU Bo ; Tang, Ming ; You, Ai-Qing ; Zhu, Li-Li ; He, Guang-Cun Topik: Agrobacterium tumefaciens; binary bacterial artificial chromosome (BIBAC); rice (Oryza sativa L.); plant transformation of large DNA fragment Bahasa: (EN )     Tahun Terbit: 2006     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: dev transf system rice -BIBAC vector.pdf (828.45KB; 2 download) Abstract An Agrobacterium-mediated transformation protocol using binary bacterial artificial chromosome (BIBAC) vector system in rice (Oryza sativa L.) was developed. Calli derived from mature embryos ofjaponica rice cv. H1493 were used as target tissues Various aspects in transformation and regeneration processes including callus induction and culture, Agrobacterium concentration and duration of co-cultivation, bacterial elimination and transformant selection were examined in order to improve the transformation efficiency. An optimized transformation conditions was established including: using an Agrobacteriurn strain, LBA4404(HP4404), which carries a super-virulent helper plasmid pCH32, for the infection; a modified N6 medium system for callus induction and culture; pH 5.6 for media in pre-cultivation and co-cultivation; Agrobacterium concentration at OD6oo = 1.0 for 3 days co-cultivation and 7 days for a resting period of the infected cab. Based on PCR and Southern blot analysis, it was demonstrated that insert DNA and marker genes carried by BIBAC2 were integrated into the rice genome. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

Copyright © 2006, 2007 Unika Atma Jaya, all rights reserved