Detail Physical and Genetic Mapping of the Rhodobacter sphaeroides 2.4.1Genome: Presence of Two Unique Circular Chromosomes (Journal of Bacteriology, Vol.171, No.11, November 1989) Bibliografi Author: Kaplan, samuel ; Suwanto, Antonius Bahasa: (EN )     Penerbit: American Society for Microbiology     Tempat Terbit: Washington, DC    Tahun Terbit: 1989     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 5850.pdf (2.9MB; 8 download) Abstract A macrorestriction map representing the complete physical map of the Rhodobacter sphaeroides 2.4.1 chromosomes has been constructed by ordering the chromosomal DNA fragments from total gesomic DNA digested with the restriction endonucleases AseI, SpeI, DraI, and SnaBI. Junction fragments and multiple restriction endonuclease digestions of the chromosomal DNAs derived from wild-type and various mutant strains, in conjunction with Southern hybridization analysis, have been used to order all of the chromosomal DNA fragments. Our results indicate that R. sphaeroides 2.4.1 carries two different circular chromosomes of 3,046 ± 95 and 914 ± 17 kilobases (kb). Both chromosome I (3,046 kb) and chromosome 1 (914 kb) contain rRNA cistrons. It appears that only a single copy of the rRNA genes is contained on chromosome I (rrnA) and that two copies are present on chromosome H (rrnB, rrnC). Additionally, genes for glyceraldehyde 3-phosphate dehydrogenase (gapB) and 8-aminolevulinic acid synthase (hemT) are found on chromosome U. In each instance, there appears to be a second copy of each of these genes on chromosome I, but the extent of the DNA homology is very low. Genes giving rise to enzymes involved in CO2 fixation and linked to the gene encoding the form I enzyme (i.e., the form I region) are on chromosome I, whereas those genes representing the form H region are on chromosome 11. The complete physical and partial genetic maps for each chromosome are presented. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

Copyright © 2006, 2007 Unika Atma Jaya, all rights reserved