Detail Genetic Transfer by Conjugation in the Thermophilic GreenSulfur Bacterium Chlorobium tepidum (Journal of Bacteriology, Vol.177, No.9, May 1995) Bibliografi Author: Madigan, Michael T. ; Wahlund, Thomas M. Topik: Thermophilic Green Sulfur Bacterium Chlorobium tepidum; Genetic Transfer Bahasa: (EN )    Edisi: May 1995     Penerbit: American Society for Microbiology     Tempat Terbit: Washington    Tahun Terbit: 1995     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: Genetic Transfer by conjugation Madigan 1995.pdf (474.64KB; 3 download) Abstract The broad-host-range IncQ group plasmids pDSK519 and pGSS33 were transferred by conjugation from Escherichia coli into the thermophilic green sulfur bacterium Chlorobium tepidum. C. tepidum exconjugants expressed the kanamycin and ampicillin-chloramphenicol resistances encoded by pDSK519 and pGSS33, respectively. Ampicillin resistance was a particularly good marker for selection in C. tepidum. Both pDSK519 and pGSS33 were stably maintained in C. tepidum at temperatures below 42&C and could be transferred between C. tepidum and E. coli without modifications. Conjugation frequencies ranged from 1021 to 1024 exconjugants per donor cell, and frequencies of 1022 to 1023 were consistently obtained when ampicillin resistance was used as a selectable marker. Methods for growth of C. tepidum on agar, isolation of plating strains and antibiotic-resistant mutants of wild-type C. tepidum cells, and optimum conditions for conjugation were also investigated. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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