Detail Oxygen as Limiting Nutrient for Growth of Cryptococcus neoformans (Journal of Clinical Microbiology, Vol.33 No.4, April 1995) Bibliografi Author: Odds, Frank C. ; Backer, Tineke de ; Dams, Gery ; Vranckx, Luc ; Woestenborghs, Filip Topik: Oxygen; Nutrient; Cryptococcus neoformans; Growth Bahasa: (EN )    Edisi: April 1995     Penerbit: American Society for Microbiology     Tempat Terbit: Washington    Tahun Terbit: 1995     Jenis: Article - diterbitkan di jurnal ilmiah internasional Fulltext: 995.pdf (157.3KB; 3 download) Abstract In microbroth cultures with RPMI 1640 medium, the growth yield of seven Cryptococcus neoformans isolates was unaffected by augmentation of the normal (0.2%) glucose concentration in the medium to 2%, and the addition of other potential carbon, nitrogen, and vitamin sources to the medium also failed to produce large changes in growth yield. However, macrobroth cultures of C. neoformans in RPMI 1640 that were agitated by rotation in air gave turbidities 6 to 37 times greater than those in identical cultures incubated statically, and similar levels of increase were seen whether the medium contained 0.2 or 2% glucose. Incubation of microplates under an oxygen atmosphere or with agitation by rotation led to an increase of up to twofold in growth turbidity of the yeast. The maximum increase was achieved by incubation with rotation and was dependent on the brand of microplate used. The findings implicate oxygen as a growth-limiting nutrient for C. neoformans. Incubation of microbroth cultures under conditions that enhance oxygen availability for antifungal susceptibility testing purposes may increase the speed of such tests and enhance the determination of MIC endpoints. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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