Analisis Marka Morfologi dan Molekuler Sifat Ketahanan Kedelai Terhadap Intensitas Cahaya Rendah  

Oleh:

Handayani, Titin ; Sastrosumarjo, Sarsidi ; Sopandie, Didy ; Suharsono ; Setiawan, Asep

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: JSTI: Jurnal Sains dan Teknologi Indonesia vol. 8 no. 1 (Apr. 2006), page 43-50.
Topik: Kedelai; Intensitas Cahaya Rendah; Morfologi; RAPD

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: SS57.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Availability of molecular marker to identify the important agronomic caharacter of plant is neede to accelerate selection activity of plant. Particularly for the complex quantitavely inherited traits-like e.g. shading tolerance of soybean- the useof such technique will speed up the process to produce adapted genotypes. The objective of this research is to identify the linkage of molecular marker RAPD with character of shading tolerance. The morphological specific characters which is corelated to shade tolerance is the number of productive branches. The intensity 75% of artificial shading is optimal level for doing selection of soybean genotypes. The inheritance of shading tolerance of soybean was controlled by gene with full dominant or by two genes pairs with dominant and recessive epistasis. There was no maternal effect in the inheritance to shade tolerance. Heritability value (0.45-0.54) indicated that the proportion variation caused by the genetic factors was moderate. Molecular analysis by using RAPD technique showed that UBC153, ROTH 480.01, and ROTH 480.03 primer have polymorphic band that can be used for inheritance study and linkage analysis. All polymorphisms segregated independently of each other. Interval mapping with Mapmaker/QTL indicated that the location of the three QTLs on linkage group were at marker tolerance locus of ROTH480.01-8125, ROTH 480.03-1125, and UBC153-19125.

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