Thermostable Chicken Feather Degrading Enzymes from L-23 Isolate from Indonesia  

Oleh:

Lintang, Rosita ; Suhartono, Maggy Thenawijaya ; Hwang, Jae-Kwan ; Pyun, Yu Ryang

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 1 no. 3 (Dec. 2007), page 109-113.
Topik: Chrysomya bezziana; Saccharomyces cerevisiae; Vaccine
Fulltext: Maggy T. Suhartono.pdf (117.22KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM78.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

The thermostable chicken feather degrading protease enzymes used here was extracted and partially purified from thermophilic bacteria L-23 isolated from a coastal hot spring in North Sulawesi, Indonesia. The L-23 was grown in the selective medium containing 1% chicken feather powder at 70 celcius degree and pH 7. The cell-free culture was precipitated with ammonium sulphate at 80% saturation, followed by heating at 65 celcius degree for 1 h before applied onto Sephadex G-100. The molecular weight of the two enzymes identified were estimated as 47 and 64 kDa. The optimum pH of the mixed enzymes preparation was 7 while the optimum temperature was 65 celcius degree. Zymogram analysis showed that one of the enzymes was still active after being heated at 100 celcius degree for 20 min and was also resistant towards organic solvents and SDS. The activity was enhanced by addition of 1 mM FeCl3.

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