Production of Recombinant Vaccine Cb Peritrophin-42 of Screwworm Fly in Escherichia coli and Saccharomyces cerevisiae  

Oleh:

Natalia, Dessy ; Muharsini, Sri ; Masduki, Fifi Fitriah ; Wardhana, April Hari ; Wardani, Savira Eka ; Maria, Elizabeth ; Heuvel, Joop Van Den

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Microbiology Indonesia vol. 1 no. 3 (Dec. 2007), page 105-108.
Topik: Chrysomya bezziana; Saccharomyces cerevisiae; Vaccine
Fulltext: Dessy Natalia.pdf (83.83KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: MM78.1 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

The screwworm fly (Chrysomya bezziana) larva is an obligate parasite which causes myasis in mammals. Vaccination is thought to be a protective and an enviromentally friendly method for combating the pest. A gene encoding a peritrophic membrane protein Cb peritrophin-42 of C. bezziana was cloned and expressed in Escherichia coli and Saccharomyces cerevisiae. Cb peritrophin-42 fused with the outer membrane protein A signal sequence was produced as an aggregate in E.coli. Expression of an Cb peritrophin-42 gene fused with oligonucleotide of the invertase signal sequence in S. cerevisiae allowed the production of 14.4 mg I soluble extracellular Cb pertrophin-42. Sheep vaccinated with recombinant Cb pertrophin-42 showed a strong immunological reaction. In vivo assay following vaccination with the recombinant Cb peritrophin-42 showed a 27% reduction in the weight of recovered larvae.

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