The Probiotic Lactobacillus acidophilus Stimulates Chloride/Hydroxyl Exchange Activity in Human Intestinal Epithelial Cells  

Oleh:

Borthakur, Alip ; Gill, Ravinder K. ; Tyagi, Sangeeta ; Koutsouris, Athanasia ; Alrefai, Waddah A.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: JN: The Journal of Nutrition vol. 138 no. 07 (Jul. 2008), page 1355.

Ketersediaan

Perpustakaan FK Nomor Panggil: J42.K.2008.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Probiotics are viable nonpathogenic microorganisms that are considered to confer health benefits to the host. Recent studies indicated that some lactobacillus function as probiotics and have been used as alternative treatments for diarrhea, which occurs due to increased secretion, decreased absorption, or both. However, the direct effects of probiotics on intestinal electrolyte absorption are not known. Therefore, we examined the effect of Lactobacillus on luminal chloride/hydroxyl (CI¯/OH¯) exchange activity in human intestinal epithelial cells. Postconfluent Caco-2 cells were treated with the Lactobacillus apecies Lactobacillus acidophilus (LA), Lactobacillus casei, Lactobacillus plantarum, or Lactobacillus rhamnosus (LR) for 3 h at a multiplicity of infection of 50. CI¯/OH¯ exchange activity was measured as 4,4'-diisothiocyanostilbene-2, 2'-disulfonic acid-sensitive 36 CI uptake in base-loaded cells. Treatment with live, but not heatkilled, LA and LR significantly increased CI¯/OH¯ exchange activity (~50%), whereas other species were inneffective. Similarly, the conditioned medium (supernatant) of live LA increased CI¯/OH¯ exchange. The ability of LA or its conditioned culture medium to enhance CI¯/OH¯activity was blocked by PI-3 kinase inhibition but was unaffected by inhibition of mitogen-activated protein kenases. Corresponding to the icreased CI¯/OH¯ exchange activity, LA treatment increased the surface expression of the apical anion exchanger, SLC26A3 [ Down Regulated in Adenoma (DRA)]. The increased DRA membrane localization might contribute to the increased CI¯ absorption by LA. Our results suggest that LA secretes soluble effector molecule(s) into the culture medium that stimulate apical CI¯/OH¯ exchange activity via phosphatidylinositol-3 kinase mediated mechanism.

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