Epigallocatechin gallate of greentea inhibits proliferation, differentiationand TNF-a in the primary human visceral preadipocytes culture  

Oleh:

Ratnawati, Retty ; Indra, M. Rasjad ; Satuman

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Majalah Ilmu Faal Indonesia vol. 06 no. 03 (Jun. 2007), page 160.
Topik: green tea epigallocatechin gallate (EGCG); human preadipocytes; proliferation; differentiation; TNF-a

Ketersediaan

Perpustakaan FK Nomor Panggil: M07.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Objectives : Green tea EGCG (epigallocatechin gallate) have shown to cause loss of body fat and also inhibit the growtg of cancer cell. Therefore the aim of this study is to determine whether green tea EGCG can directly inhibit proliferation, differentiation and TNF-a (tumor necrosis factor-a) of the human visceral preadipocyte culture. Methods : Primary human visceral pre adipocytes culture were treated with EGCG in several doses o uM (control group), 10uM, 50 uM and 200 uM for 24 hours respectively. the human visceral tissues specimens were derived from the surgery dept. of Dr. Syaiful Anwar General HOspital in Malang. Cellular account of the adipogenesis processess (proliferation, differentiated, and undifferentiated cells) was determined using microscope after hematoxylline staining. TNF-a consenteration derived from primary human preadipocytes cultur also observed using ELISA Kit Assay design. Result : It was found that there were a dose dependent respond to the cellular account of the adipogenesis processes. There were a significant (p=0.000) decrease of the cellular account for proliferation in EGCG control group (78.8 ± 5.88%), EGCG 10 uM (66.32 ± 4.63%), EGCG 50 uM (48.72 ± 3.84%), EGCG 100 uM (28.2 ± 4.63%) and EGCG 200 uM (62.68 ± 1.24%). there were not any changes in the percentages of undifferentiation cell in the group of EGCG 10 uM and 50 uM, however there was significant (p<0.001) increase in EGCG 100 uM (78.47 ± 3.44%) and EGCG 200 uM (71.95 ± 1.24%) compared to ECGC control group (33.56 ± 3.62%). The consentration of TNF-a also decrease in the dose dependent of EGCG. It was showed that the EGCG dose 100 uM yielded maximum efect in the inhibition of the adipogenesis process. Conclusions : Green tea EGCG inhibits adipogenesis processes in the level of proliferation, differentiation cells and TNF-a, in the dose dependent manner.

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