Pola Mutan Gen Glukosa 6 Fosfat dehidrogenase di Surabaya  

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Suhartati

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI
Dalam koleksi: Majalah Ilmu Faal Indonesia vol. 06 no. 03 (Jun. 2007), page 130.
Topik: G6PD deficiency; MPTP; history of miscarriages; oxidants

Ketersediaan

Perpustakaan FK Nomor Panggil: M07.K Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Glukosa 6 Fosfat dehidrogenase (G6PD) deficiency was studied in open Surabaya region. Seventy-six blood samples were collected from Surabaya. The samples were screened using Randox kit to determine the activity of G6PD, taking a cut-off value of less than 60% as indicating G6PD deficiency. Thirty one of cases tested positive with the Randox kit test, 26 of which tested positive with MPTP. In this study, genomic DNA from blood samples were extracted using simple rapid genomic DNA method. The extracted DNA was then used to amplify exon 5, 6, 9, 11 and 12 of the G6PD genes to detect mutans using Multiplex PCR with Multiple Tandem forward Primers and a common reverse primer (MPTP) technique according to Shirakawa. Seven different of G6PD mutants were found in Surabaya : 4 cases of Vanua Lava, a single G6PD Viangchan cases, 5 cases of G6PD Chatam, 2 cases of G6PD union, 7 cases of canton, 6 cases of Kaiping, and 2 cases of silent mutation. Double mutants were also found, all involving silent mutations : Canton + silent, Vanua + silent, and viangchan + silent, one case each. Nine cases of G6PD deficiency were found in women with a history of miscarriages : 3 cases of Canton, 3 cases of Kaiping, one single case of Vanua Lava, and 2 tested negative with MPTP. G6PD deficiency is usually asymptomayic. Symptoms will only appear if those person ingest oxidants such as certain drugs or foodstuffs. THe disorders manifest itself as hemolytic anemia, repeated hepatitis, repeated miscarriages etc. Since G6PD deficiency is more or less prevalent in Indonesia, an early diagnosis would be of advantage, so that people with G6PD deficiency could avoid ingesting oxidants.

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