Comparison of MacConkey, Salmonella-Shigella, and xylose lysine deoxycholate agar for isolation of Shigella from rectal swab of diarrheal patients  

Oleh:

Suryawidjaja, Julius E. ; Salim, Oktavianus Ch. ; Bukitwetan, Paul ; Lesmana, Murad

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI - non-atma jaya
Dalam koleksi: Universa Medicina vol. 26 no. 02 (Apr. 2007), page 57.

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Perpustakaan FK Nomor Panggil: U01.K.01 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

BACKGROUND Generall, isolation of Shigella from stool specimen required differential and selective media such as MacConkey (MAC), Salmonella-Shigella (SS), xylose-lysine-deoxycholate (XLD), and Hektoen enteric (HEA). To obtain high recovery rate of enteric pathogens, a combination of moderately inhibitive and highly selective media is used. Unfortunately, none of these media were choose as the best media by clinical laboratories. The objective of this study was to compare MAC, SS, and XLD media to determine its sensitivity for isolating Shigella. METODE Rectal swab from diarrheal patients was cultured on MAC, SS and XLD agar and the plates were incubated at 370C, for 24 hr. Suspected Shigella colonies appeared as non-lactose fermenting were selected and subcultured in biochemical media for the identification. Serologic test for confirmation of Shigella identification was performed by using specific anti-sera from DIFCO (Difco laboratories, Detroit, MI). A software package, Epi Info version 6, (Center for Disease Control and Prevention) was used for statistical analysis. RESULTS A total of 1027 rectal swabs from diarrheal patients were obtained and cultured on MAC, SS, and XLD agar. Overall, Shigella was isolated from 8.4% specimens, comprised of S. flexneri, 6.2%, S. sonnei, 1.9%, S. boydii, 0.2% and S. dysenteriae, 0.2%. The isolation rate for Shigella on MAC was 5.1%, on SS was 4.8%, and on XLD was 7.1%. Combination of media showed that MAC+SS yield 6.5% of Shigella isolates, MAC+XLD 8.1%, and SS+XLD 7.9%. Out of 86 positive rectal swab samples for Shigella, 20 (22.7%) isolates were recovered from XLD only, 5 (5.8%) from SS only and 6 (7.0%) from MAC only. CONCLUSION For isolation of S. flexneri and S. sonnei, XLD was the most sensitive media. Results of the MAC+XLD was the best differential combination and selective media for maximum isolation of Shigella.

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