Deteksi Molekular Bakteri Endofit pada Jaringan Planlet Tebu  

Oleh:

Widayati, Wiwik E. ; Widada, Joko ; Soedarsono, Joedoro

Jenis: Article from Journal - ilmiah nasional - terakreditasi DIKTI
Dalam koleksi: Hayati Journal of Biosciences vol. 14 no. 4 (Dec. 2007), page 145-149.
Topik: Endophytic Bacteria; PCR; RIS; 16S rDNA; SSCP
Fulltext: 122.pdf (57.77KB)

Ketersediaan

Perpustakaan Pusat (Semanggi) Nomor Panggil: HH13.9 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

Endophytic bacteria live in plant host tissue without causing any symptoms. The aim of this study was to examine the indigenous endophytic bacteria on sugarcane plantlets produced from the young leaf cells by using tissue culture techniques. To detect the existence of endophytic bacteria in the plantlet tissue, a series of molecular method based on PCR were applied by using ribosomal intergenic spacer (RIS) primer followed by 16S rDNA partial sequence and single strand conformation polymophism (SCCP). The results showed that the molucular method could detect the existence of bacteria in the tissue. Using the same methods, the bacteria were also found in other developmental stages of sugarcane (explants, differentiated tissues and callus).

Opini Anda

Klik untuk menuliskan opini Anda tentang koleksi ini!

Kembali