Detail PROFIL RESPIRASI SELAMA PROSES PEMBUATAN KULTUR SEL KEDELAI (Glycine max. L) Bibliografi Author: Djajanegara, Ira ; Day, David (Co-Author) Topik: AOX; COX; suspension cell Bahasa: (ID )     Penerbit: Perhimpunan Bioteknologi Pertanian Indonesia     Tempat Terbit: Malang    Tahun Terbit: 2005     Jenis: Papers/Makalah - pada seminar nasional Fulltext: Makalah-Ira Jayanegara.pdf (130.12KB; 0 download) Abstract There are 3 phases during the development of soybean suspension cell culture. Those phases are leaf, callii and suspension cells. During the development process, the respiration through cytochrome (COX) pathway and alternative (AOX) pathway was monitored and compared. Respiration were measured at tissue level in which respiration of the whole tissue was monitored and at mitochondrial level in which the respiration of the isolated mitochondria was monitored. Whole cells respiration through cytochrome pathway (COX) and alternative (AOX) pathway were also monitored during 7 days in culture which is a period for subsequent subculture. In this experiment cytochrome pathway respiration is more dominant than alternative pathway respiration at leaf and suspension cell phases whereas the alternative pathway respiration is more dominant at the suspensiion cell phase. This indicates that under a certain condition respiration through the alternative pathway can be the main operating pathway of the mitochondrial electron transport chain. Whole cell respiration during a week culture period showed a peak at 3 days after subculture (DAS) whereas for alternative pathway the peak was reached at 2 days after subculture (DAS) Immunoblot of AOX proteins from several tissues of soybean indicates that there were 2 isoforms of alternative oxidase proteins obtained from mitochondria isolated from leaf and cotyledons whereas immunnoblot from mitochondria isolated from root, callii and suspension cells showed only 1 isoform of AOX proteins. This experiment indicates that eventhough immunoblot showed the abundance of the AOX proteins, the respiration through AOX pathway was not correlated. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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