Detail High-Resolution Crystal Structure of an Engineered Human ß2-Adrenergic G Protein–Coupled Receptor   Oleh:  Cherezov, Vadim ; Rosenbaum, Daniel M. ; Hanson, Michael A. ; Rasmussen, Soren G.F. ; Foon, Sun Thian ; Tong, Sun Kobi ; Hee-Jung, Choi ; Kuhn, Peter ; Weis, William I. ; Kobilka, Brian K. ; Stevens, Raymond C. Jenis: Article from Bulletin/Magazine Dalam koleksi: SCIENCE (keterangan: ada di Proquest) vol. 318 no. 5854 (Nov. 2007), page 1258. Ketersediaan Perpustakaan FK Nomor Panggil: S01.K.2007.09 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada     Lihat Detail Induk Isi artikel Heterotrimeric guanine nucleotide–binding protein (G protein)–coupled receptors constitute the largest family of eukaryotic signal transduction proteins that communicate across the membrane. We report the crystal structure of a human ß2-adrenergic receptor–T4 lysozyme fusion protein bound to the partial inverse agonist carazolol at 2.4 angstrom resolution. The structure provides a high-resolution view of a human G protein–coupled receptor bound to a diffusible ligand. Ligand-binding site accessibility is enabled by the second extracellular loop, which is held out of the binding cavity by a pair of closely spaced disulfide bridges and a short helical segment within the loop. Cholesterol, a necessary component for crystallization, mediates an intriguing parallel association of receptor molecules in the crystal lattice. Although the location of carazolol in the ß2-adrenergic receptor is very similar to that of retinal in rhodopsin, structural differences in the ligand-binding site and other regions highlight the challenges in using rhodopsin as a template model for this large receptor family. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Kembali

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