Detail Detection of Genes Encoding Starch-degrading Enzymes from Jae Sawn Hot Spring in Thailand Bibliografi Author: Waturangi, Diana Elizabeth (Advisor); Kristina, Puji Topik: Enzim; Sumber Air Panas Bahasa: (EN )     Penerbit: Fakultas Teknobiologi Unika Atma Jaya (Faculty of Biotechnology Atma Jaya Catholic University of Indonesia)     Tempat Terbit: Jakarta    Tahun Terbit: 2006     Jenis: Theses - Undergraduate Thesis Fulltext: Puji Kristina's Undergraduated Theses .pdf (80.16KB; 19 download) Puji Kristina Undergraduated Theses.pdf (596.72KB; 6 download) Ketersediaan Perpustakaan Pusat (Semanggi) Nomor Panggil: FTB-006 Non-tandon: tidak ada Tandon: 1  Lihat Detail Induk Abstract Sumber air panas merupakan salah satu habitat mikroorganisme penghasil enzim yang stabil pada suhu tinggi. DNA metagenomic dan sumber air panas Jae Sawn di Thailand diisolasi dan digunakan sebagai cetakan untuk deteksi gen neopullulanase dan a-amilase. Fragmen internal sepanjang 552 pasang basa yang mengandung empat daerah konservatif gen-gen tersebut berhasil diamplifikasi dengan PCR menggunakan primer degenerate. Dua modifikasi PCR digunakan untuk melakukan amplifikasi daerah hulu dan hilir fragmen internal atau dikenal sebagai metode genome walking. Dengan metode Adaptor-mediated genome walking, daerah hulu sepanjang 30 pasang basa dan daerah hilir sepanjang 10 pasang basa gen neopullulanase berhasil disekuens. Dengan metode Semi-Random Two-Stepped PCR. daerah hulu gen neopullulanase sepanjang 950 pasang basa berhasil disekuens dan daerah hulu gen aamilase sepanjang 900 pasang basa yang mengandung informasi start codon berhasil disekuens. Hot spring has been known as a reservoir of thermo stable enzymes produced by microorganisms. Metagenomic DNA from Jae Sawn hot spring in Thailand was isolated and used as a PCR template for neopullulanase and a-amylase genes detection. Internal fragments with 552bp length containing four conserved regions of the genes were amplified using degenerate primers. Two PCR modifications were used to amplify upstream and downstream flanking regions of the internal fragments, known as genome walking. With Adaptor-mediated genome walking, upstream flanking region of neopullulanase gene was obtained with 3Obp length and the downstream flanking region was obtained with lObp length. Using Semi-Random Two-Stepped PCR, 950bp fragment of neopullulanase upstream flanking region and 900bp fragment of a-amylase upstream flanking region were obtained. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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