Transgenic Strategies for Combinatorial Expression of Fluorescent Proteins in The Nervous System  

Oleh:

Livet, Jean ; Weissman, Tamily. A. ; Kang, Hyuno ; Draft, Ryan. W. ; Ju, Lu ; Bennis, Robyn. A. ; Sanes, Joshua. R. ; Lichtman, Jeff. W.

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: NATURE (keterangan: ada di Proquest) vol. 450 no. 7166 (Nov. 2007), page 56.

Ketersediaan

Perpustakaan FK Nomor Panggil: N01.K.2007.10 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Isi artikel

Detailed analysis of neuronal network architecture requires the development of new methods. Here we present strategies to visualize synaptic circuits by genetically labelling neurons with multiple, distinct colours. In Brainbow transgenes, Cre/lox recombination is used to create a stochastic choice of expression between three or more fluorescent proteins (XFPs). Integration of tandem Brainbow copies in transgenic mice yielded combinatorial XFP expression, and thus many colours, thereby providing a way to distinguish adjacent neurons and visualize other cellular interactions. As a demonstration, we reconstructed hundreds of neighbouring axons and multiple synaptic contacts in one small volume of a cerebellar lobe exhibiting approximately 90 colours. The expression in some lines also allowed us to map glial territories and follow glial cells and neurons over time in vivo. The ability of the Brainbow system to label uniquely many individual cells within a population may facilitate the analysis of neuronal circuitry on a large scale.

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