Detail Torreyanic acid: Affinity chromatographic identification of receptors and biochemical analysis of the torreyanic acid-eIF-4A complex Bibliografi Author: Justman, Craig Jerome ; Schreiber, Stuart L. (Advisor) Topik: CHEMISTRY; BIOCHEMISTRY Bahasa: (EN )    ISBN: 0-599-77652-8     Penerbit: Harvard University Press     Tahun Terbit: 2000     Jenis: Theses - Dissertation Fulltext: 9972343.pdf (0.0B; 0 download) Abstract Affinity chromatography was used to identify binding proteins for two natural products: brefeldin A and torreyanic acid. Evidence is presented that brefeldin A binds to two proteins not related to its classic biological activity in protein secretion: crm1/xpoI and L-type calcium channel α 2-subunit. The possible consequences of these interactions are disscussed. Several proteins are identified based on specific binding to a torreyanic acid affinity reagent. The eukaryotic translation initiation factor 4A (eIF-4A) was identified as a probable effector molecule of torreyanic acid action. (eIF-4A) is an RNA-dependent helicase from the DEAD box protein family. It is required for cap-dependent and cap-independent translation. Evidence for a covalent torreyanic acid-eIF-4A adduct is presented. Biochemical analysis of torreyanic acid-eIF-4A complex was performed in vitro and in vivo. Torreyanic acid was shown to inhibit the ATPase, ATP-binding and RNA-binding activities of eIF-4A. A consequence of this interaction is that under specific conditions, torreyanic acid both inhibits and stimulates translation in vivo and in vitro. A model for this dualism is presented. Torreyanic acid is the first identified endogenous ligand for eIF-4A and small molecule effector or translation initiation. Torreyanamide, the bis-diethylamide of torreyanic acid, was synthesized. It has the same biological profile as torreyanic acid, but is more potent. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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