Detail The effect of mass transfer and hydrodynamics on fluidized bed adsorption of proteins Bibliografi Author: Wright, Pamela R. ; Muzzio, Fernando J. (Advisor); Glasser, Benjamin J. (Advisor) Topik: ENGINEERING; CHEMICAL|CHEMISTRY; ANALYTICAL Bahasa: (EN )    ISBN: 0-599-61715-2     Penerbit: RUTGERS THE STATE UNIVERSITY OF NEW JERSEY - NEW BRUNSWICK     Tahun Terbit: 2000     Jenis: Theses - Dissertation Fulltext: 9958492.pdf (0.0B; 3 download) Abstract Fluidized bed adsorption systems have recently been used for the primary recovery of therapeutic and drug products from fermentation broths. This primary recovery technique offers the advantage of performing product capture, feedstock clarification and product concentration in a single step. Although the feasibility of fluidized bed adsorption has been demonstrated, the combined effects of intraparticle mass transfer resistance and the bulk phase hydrodynamics remain unresolved. Since many of the key parameters such as axial dispersion, superficial velocity, film mass transfer and solid diffusion are inter-related it is not possible to experimentally isolate and quantify each effect on the fluidized bed adsorption process. As a result, the performance of fluidized bed adsorption cannot be accurately predicted. The performance of chromatographic resins used for adsorption of proteins is dependent on several factors including solid and liquid phase diffusivity, film and intraparticle mass transfer effects. In this dissertation, the effects of intraparticle mass transfer resistances were investigated using two commercially available cation exchange resins, Streamline SP and S-HyperD LS. Streamline SP is a macroporous, resin and mass transfer in the particle is well described by pore diffusion. S-HyperD LS is a gel composite resin and mass transfer in the resin is by solid diffusion. Initially, mass transfer rates of each resin were characterized by measuring uptake of lysozyme in viscous and non-viscous solutions in stirred vessels. Solid phase diffusion coefficients obtained from simulations were in agreement with published results for macroporous and hyper-diffusive particles. The effect of particle size distributions on batch adsorption was investigated and found to have a negligible contribution to uptake rates compared to the effect of an average particle size. Fluidized bed adsorption experiments were performed to characterize adsorption as a function of mass transfer and solution viscosity for each resin. Using experimentally derived parameters for axial dispersion, solid dispersion and bed expansion, a model was developed and solved to describe protein adsorption in a fluidized bed. Liquid-solid mass transfer, adsorption and hydrodynamic effects were taken into account for both pore and homogeneous diffusion. Parametric sensitivity analysis showed that superficial velocity and particle radius had the largest effects on breakthrough behavior for all conditions. The simulation results for pore diffusion were more significantly affected by changes in superficial velocity and particle radius than the simulation results for homogeneous diffusion. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Lihat Sejarah Pengadaan  Konversi Metadata   Kembali

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