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BukuA study on the fundamentals and development of fungal bioreactors for kraft mill streams and other applications
Bibliografi
Author: Pallerla, Sammaiah ; Chambers, Robert P. (Advisor)
Topik: ENGINEERING; CHEMICAL|ENGINEERING; ENVIRONMENTAL
Bahasa: (EN )    ISBN: 0-591-37279-7    
Penerbit: AUBURN UNIVERSITY     Tahun Terbit: 1997    
Jenis: Theses - Dissertation
Fulltext: 9728115.pdf (0.0B; 2 download)
Abstract
A new continuous fluidized bed bioreactor system containing polyurethane immobilized white-rot fungus Trametes versicolor was developed which successfully decolorized and dechlorinated kraft bleach plant effluents. The polymer-immobilized biocatalyst was robust with respect to maintaining biological activity and mechanical stability for 32 days in continuous operation. The biological and mechanical stability for this system was significantly greater than that for the calcium alginate immobilized fungal system. The fluidized bioreactor using polyurethane immobilized T. versicolor shows considerable promise for application as a biotreatment process for decolorization and dechlorination of bleach plant effluents. The color reduction reaction was found to obey first order kinetics in the fluidized bioreactor. The rate of color reduction was linearly dependent on the bioreactor inlet glucose concentration up to a level of 0.8 wt.%. The performance of the bioreactor was significantly enhanced in an oxygen atmosphere. The fungal bioreactor fluidized with oxygen could handle effluent of higher strength with sustained performance than bioreactor fluidized with air. Experimental data were obtained by growing the fungus under static and shake culture conditions. The growth kinetic models of Monod, Williams and Emerson were examined and the growth kinetic parameters were estimated. A study was conducted to investigate the combined treatment and potential internal utilization of methanol from foul condensate. The results indicated that simultaneous treatment of caustic bleach filtrate and foul condensate by immobilized T. versicolor in a fluidized bed bioreactor is effective in reducing color, AOX and methanol. Application of the fluidized fungal bioreactor for biodegradation of pentachlorophenol (PCP) was investigated in batch and continuous bioreactor studies. Results with both active and deactivated fungus showed that the degradation process proceeds intracellularly after initial uptake by the mycelia. The PCP biodegradation reaction rates were determined in the fluidized bioreactor and these values were compared with computer simulation of the simultaneous reaction and mass transfer occurring within the immobilized fungus. The results indicated that the fungus immobilized in polyurethane particles have only a slight diffusional resistances for PCP, oxygen and glucose.
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