THE EFFECT OF RED FRUIT OIL (Pandanus conoideus) ON OXIDATIVE STRESS OF ENDOTHELIAL CELLS EXPOSED TO MALARIA FALCIPARUM PATIENT SERUM AND HEALTHY DONOR NEUTROPHIL  

Oleh:

Yunita Armiyanti ; Loeki Enggar Fitri ; Edi Widjajanto

Jenis: Article from Journal - ilmiah nasional
Dalam koleksi: Jurnal Kedokteran Brawijaya vol. 23 no. 01 (Apr. 2007), page 6.
Topik: Plasmodium falciparum; endothelial cells; ROI; red fruit oil

Ketersediaan

Perpustakaan FK Nomor Panggil: J34.K.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

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Complication in Plasmodium falciparum malaria is associated with endothelial damage and overproduction of free radicals (oxidative stress) by activated neutrophils and endothelial cells. Red fruit (Pandanus conoideus) oil has a very high antioxidant content, therefore it may neutralize free radicals and prevent endothelial damage. The aim of this research is to prove that red fruit (Pandanus conoideus) oil reduce Reactive Oxygen Intermediate (ROI) production of endothelial cells exposed to severe malaria patient serum and neutrophils from healthy donor. Endothelial cells from human umbilical veins were coincubated with serum from severe malaria patient and with neutrophils from healthy donor (positive control group). HUVEC's normal was used as negative control, where as the experimental groups were given with red fruit oil in different concentrations (2.8%, 5.7% dan 11.3%). The Reactive Oxygen Intermediate (ROI) production of endothelial cells. was semiquantitative/y measured by using NBT-reduction assay and the score of ROI was counted. The results were statistically analyzed with ANNOVA (p<0.01). The rate of ROI production of endothelial cells was markedly increased after incubation with patient serum and neutrophils. The red fruit oil in different concentrations could significantly decrease the rate of ROI production of cells. There was negative correlation between red fruit oil concentration with ROI prodvction (Spearman's analyzed, p<0.01, Correlation Coeff = -0.950), ROI score (Correlation Coeff =. ~0.928). It is concluded that red fruit (Pandanus conoideus) oil neutrslized oxidative stress of cells by reducing ROI production of endothelial cells exposed to severe malaria patient serum and neutrophils from healthy donor in vitro.

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