Sintesis dan Amplifikasi cDNA Penyandi Protein GRA1 Takizoit Toxoplasma Gondii Isolat Lokal  

Oleh:

Erma Sulistyaningsih ; Artama, Wayan T.

Jenis: Article from Journal - ilmiah nasional - tidak terakreditasi DIKTI - atma jaya
Dalam koleksi: Majalah Kedokteran Damianus vol. 06 no. 03 (Sep. 2007), page 171-176.
Topik: GRA1 Protein; Toxoplasma Gondii; Tachyzoite; Complementary DNA
Fulltext: D01 v6 n3 p171 kelik2023.pdf (972.79KB)

Ketersediaan

Perpustakaan PKPM Nomor Panggil: M61 Non-tandon: 2 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk
Perpustakaan FK Nomor Panggil: D01.K.2007.01 Non-tandon: 3 (dapat dipinjam: 0) Tandon: tidak ada	Lihat Detail Induk

Isi artikel

The aim of the research was to synthesis and amplifY the cDNA encoding GRAJ protein oftachyzoite Toxoplasma gondii local isolate by DNA recombinant technology. Tachyzoite was grown in Balble mice in vivo. Total RNA was isolated using RNAgents Total RNA Isolation System (Promega). Messenger RNA was isolated ji'om total RNA using PolyATract mRNA Isolation System (Promega) and it was used to synthesis cDNA using Universal Riboclone cDNA Synthesis System (Promega). Complementary DNA was amplified using PuRe Taq RTG-PCR Beads (Amersham Bioscience) with specific primers (CybergeneAB). The amplified DNA fragment was electroforated on a 1 % agarose gel (SeaKem). The result showed that amplification of cDNA using specific primer of GRA J produced+- 700 bp fragment of gene encoding GRA I protein of Toxoplasma gondii local isolate.

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