Detail Redirection of Silencing Targets by Adenosine-to-Inosine Editing of miRNAs   Oleh:  Kawahara, Yukio ; Zinshteyn, Boris ; Sethupathy, Praveen ; Lizasa, Hisashi ; Hatzigeorgiou, Artemis G. ; Nishikura, Kazuko Jenis: Article from Bulletin/Magazine Dalam koleksi: SCIENCE (keterangan: ada di Proquest) vol. 315 no. 5815 (Feb. 2007), page 1137. Topik: Molecular biology Ketersediaan Perpustakaan FK Nomor Panggil: S01.K.2007.02 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada     Lihat Detail Induk Isi artikel Primary transcripts of certain microRNA (miRNA) genes are subject to RNA editing that converts adenosine to inosine. However, the importance of miRNA editing remains largely undetermined. Here we report that tissue specific adenosine-to-inosine editing of miR-376 duster transcripts leads to predominant expression of edited miR-376 isoform RNAs. One highly edited site is positioned in the middle of the 5 proximal half "seed" region critical for the hybridization of miRNAs to targets. We provide evidence that the edited miR-376 RNA silences specifically a different set of genes. Repression of phospho ribosyl pyrophosphate synthetase 1, a target of the edited miR-376 RNA and an enzyme involved in the uric-acid synthesis pathway, contributes to tight and tissue-specific regulation of uric-acid levels, revealing a previously unknown role for RNA editing in miRNA-mediated gene silencing. Opini Anda Klik untuk menuliskan opini Anda tentang koleksi ini! Kembali

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