MAPK-mediated bimodal gene expression and adaptive gradient sensing in yeast  

Oleh:

Paliwal, Saurabh ; Iglesias, Pablo A ; Campbell, Kyle ; Hilioti, Zoe ; Groisman, Alex ; Levchenko, Andre

Jenis: Article from Journal - ilmiah internasional
Dalam koleksi: NATURE (keterangan: ada di Proquest) vol. 446 no. 7131 (Mar. 2007), page 46.

Ketersediaan

Perpustakaan FK Nomor Panggil: N01.K.2007.03 Non-tandon: 1 (dapat dipinjam: 0) Tandon: tidak ada

Lihat Detail Induk

Isi artikel

The mating pathway in Saccharomyces cerevisiae has been the focus of considerable research effort, yet many quantitative aspects of its regulation still remain unknown. Using an integrated approach involving experiments in microfluidic chips and computational modelling, we studied gene expression and phenotypic changes associated with the mating response under well-defined pheromone gradients. Here we report a combination of switch-like and graded pathway responses leading to stochastic phenotype determination in a specific range of pheromone concentrations. Furthermore, we show that these responses are critically dependent on mitogen-activated protein kinase (MAPK)-mediated regulation of the activity of the pheromone-response-specific transcription factor, Ste12, as well as on the autoregulatory feedback of Ste12. In particular, both the switch-like characteristics and sensitivity of gene expression in shmooing cells to pheromone concentration were significantly diminished in cells lacking Kssl, one of the MAP kinases activated in the mating pathway. In addition, the dynamic range of gradient sensing of Kssl-deficient cells was reduced compared with wild type. We thus provide unsuspected functional significance for this kinase in regulation of the mating response.

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